GTDB Forum

Root of bacterial tree?

Hello,

What is considered to be the root of the bacterial tree for GTDB release 202?

Thanks,

Kate Shulgina

Hi. The GTDB reference tree should be treated as unrooted. If your question is in regards to calculating RED, we use an operational definition which uses the median RED value over a set of plausible rootings (see https://www.nature.com/articles/nbt.4229 for details).